2021

Importance of protein intrinsic conformational dynamics and transient nature of non-covalent interactions in ligand binding affinity.
International Journal of Biological Macromolecules 192 (2021), 692-700, doi:10.1016/j.ijbiomac.2021.10.045
Mario Kekez, Vladimir Zanki, Ivan Antičević, Jasmina Rokov Plavec, Aleksandra Maršavelski

The evolutionary history of the HUP domain.
Critical Reviews in Biochemistry and Molecular Biology (2021), doi:10.1080/10409238.2021.1957764
Ita Gruic-Sovulj, Liam M. Longo, Jagoda Jablonska, Dan S. Tawfik

2020

Polyamines Mediate Folding of Primordial Hyperacidic Helical Proteins.
Biochemistry. 59 (2020), 4456-4462, doi:10.1021/acs.biochem.0c00800
Dragana Despotović, Liam M. Longo, Einav Aharon, Amit Kahana, Tali Scherf, Ita Gruic-Sovulj, Dan S. Tawfik

How evolution shapes enzyme selectivity – lessons from aminoacyl‐tRNA synthetases and other amino acid utilizing enzymes.
The FEBS Journal. 287 (2020), 1284-1305, doi:10.1111/febs.15199
Dan S. Tawfik, Ita Gruic-Sovulj

Mechanism of discrimination of isoleucyl‐tRNA synthetase against non‐proteinogenic α‐amino butyrate and its fluorinated analogues.
The FEBS Journal. 2020, doi:/10.1111/febs.15053
Igor Zivkovic, Johann Moschner, Beate Koksch, Ita Gruic Sovulj.

2019

On the Mechanism and Origin of Isoleucyl-tRNA Synthetase Editing against Norvaline.
Journal of Molecular Biology. 2019, doi:10.1016/j.jmb.2019.01.029
Bilus Mirna, Semanjski Maja, Mocibob Marko, Zivkovic Igor, Cvetesic Nevena, Tawfik S. Dan, Toth-Petroczy Agnes, Macek Boris, Gruic-Sovulj Ita.

Arabidopsis seryl‐tRNA synthetase: the first crystal structure and novel protein interactor of plant aminoacyl‐tRNA synthetase.
The FEBS Journal. 286 (2018), 3; 536-554
Kekez Mario; Zanki Vladimir; Kekez Ivana; Baranasic Jurica; Hodnik Vesna; Duchene Anne-Marie; Anderluh Gregor; Gruic Sovulj Ita; Matković-Čalogović Dubravka; Weygand Durasevic Ivana; Rokov-Plavec Jasmina.

2018

Kinetic Origin of Substrate Specificity in Post-Transfer Editing by Leucyl-tRNA Synthetase.
Journal of Molecular Biology. 430 (2018), 1; 1-16
Dulic Morana, Cvetesic Nevena, Zivkovic Igor, Palencia Andrés, Cusack Stephen, Bertosa Branimir, Gruic-Sovulj Ita.

An archaeal aminoacyl-tRNA synthetase complex for improved substrate quality control.
Biochimie. 147 (2018), 1; 36-45
Crnković Ana, Čavužić Mirela, Godinić-Mikulčić Vlatka, Anderluh Gregor, Weygand-Đurašević Ivana, Gruić-Sovulj Ita.

2017

Discovery and Investigation of Natural Editing Function against Artificial Amino Acids in Protein Translation.
ACS Central Science. 3 (2017), 1; 73-80
Voeller Jan-Stefan; Dulic Morana; Gerling-Driessen Ulla I. M.; Biava Hernan; Baumann Tobias; Budisa Nediljko; Gruic-Sovulj Ita; Koksch Beate

Synthetic and editing reactions of aminoacyl-tRNA synthetases using cognate and non-cognate amino acid substrates.
Methods. 113 (2017), 13-26
Cvetesic Nevena; Gruic-Sovulj Ita.

2016

Proteome-wide measurements of non-canonical mistranslation by quantitative mass spectrometry of protein modifications.
Scientific Reports. 6:28631.
Cvetesic Nevena; Semanjski Maja; Soufi Boumediene; Krug Karsten, Gruic-Sovulj Ita; Macek Boris.

Naturally Occuring Isoleucyl-tRNA Synthetase without tRNA-dependent Pre-transfer Editing.
The Journal of biological chemistry. 291 (2016), 16; 8618-8631 
Cvetesic Nevena; Dulic Morana; Bilus Mirna; Sostaric Nikolina; Lenhard Boris; Gruic-Sovulj Ita.

Exclusive cytosolic localization and broad tRNA^Ser specificity of Arabidopsis thaliana seryl-tRNA synthetase.
Journal of plant biology. 59 (2016), 1; 44-54
Kekez Mario; Bauer Natasa; Saric Ela; Rokov- Plavec Jasmina.

Seryl-tRNA Synthetases in Translation and Beyond
Croatica Chemica Acta. 89 (2016), 2; 261-276
Mocibob Marko; Rokov-Plavec Jasmina; Godinic-Mikulcic Vlatka; Gruic-Sovulj Ita.

2015

The origin of specificity and insight into recognition between an aminoacyl carrier protein and its partner ligase.
Physical Chemistry Chemical Physics. 17 (2015), 29; 19030-19038
Marsavelski Aleksandra; Mocibob Marko; Gruic-Sovulj Ita; Vianello Robert.

The tRNA A76 Hydroxyl Groups Control Partitioning of the tRNA-dependent Pre- and Post-transfer Editing Pathways in Class I tRNA Synthetase.
The Journal of biological chemistry. 290 (2015), 22; 1398-1391 
Cvetesic Nevena; Bilus Mirna; Gruic-Sovulj Ita.

2014

The physiological target for LeuRS translational quality control is norvaline. 
EMBO journal. 33 (2014), 15; 1639-1653
Cvetesic Nevena; Palencia Andres; Halasz Ivan; Cusack Stephen; Gruic-Sovulj Ita.

Determinants for tRNA-dependent pre-transfer editing in the synthetic site of isoleucyl-tRNA synthetase.
Biochemistry. 53 (2014), 39; 6189-6198
Dulic Morana; Perona John Joseph; Gruic Sovulj Ita.

Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs.
Nucleic acids research. 42 (2014), 8; 5191-5201 
Godinic-Mikulcic Vlatka; Jaric Jelena; Greber J Basil; Franke Vedran; Hodnik Vesna; Anderluh Gregor; Ban Nenad; Weygand- Durasevic Ivana.

Synthetic and Editing Mechanisms of Aminoacyl-tRNA Synthetases. 
Topics in current chemistry. 344 (2014) ; 1-42 
Perona John J; Gruic-Sovulj Ita.

A single amino acid substitution affects the substrate specificity of the seryl-tRNA synthetase homologue.
Molecular biosystems. 10 (2014), 12; 3207-3216
Marsavelski Aleksandra; Lesjak Sonja; Mocibob Marko; Weygand-Durasevic Ivana; Tomic Sanja.

2013

Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation.
Structure. 21 (2013), 4; 614-626
Mocibob Marko; Ivic Nives; Luic Marija; Weygand-Durasevic Ivana.

Lack of discrimination against non-proteinogenic amino acid norvaline by elongation factor Tu from Escherichia coli. 
Croatica chemica acta. 86 (2013), 1; 73-82 
Cvetesic Nevena; Akmacic Irena; Gruic-Sovulj Ita.

Substrate recognition and fidelity of maize seryl-tRNA synthetases. 
Archives of biochemistry and biophysics. 529 (2013), 2; 122-130
Rokov-Plavec Jasmina; Lesjak Sonja; Gruic- Sovulj Ita; Mocibob Marko; Dulic Morana; Weygand-Durasevic Ivana.

2012

Kinetic Partitioning between Synthetic and Editing Pathways in Class I Aminoacyl-tRNA Synthetases Occurs at Both Pre-transfer and Post-transfer Hydrolytic Steps.
The Journal of biological chemistry. 287 (2012), 30; 25381-25394
Cvetesic, Nevena; Perona, John J.; Gruic-Sovulj, Ita.

Arginyl-tRNA Synthetase Facilitates Complex Formation Between Seryl-tRNA Synthetase and its Cognate Transfer RNA.
Croatica chemica acta. 85 (2012) , 4; 441-449
Godinic-Mikulcic Vlatka; Jaric Jelena; Weygand-Durasevic Ivana.

Cryo-EM Structure of the Archaeal 50S Ribosomal Subunit in Complex with Initiation Factor 6 and Implications for Ribosome Evolution.
Journal of molecular biology. 418 (2012), 3/4; 145-160
Greber Basil J; Boehringer Daniel; Godinic-Mikulcic Vlatka; Crnkovic Ana; Ibba Michael; Weygand-Durasevic Ivana; Ban Nenad.

2011

An idiosyncratic serine ordering loop in methanogen seryl-tRNA synthetases guides substrates through seryl-tRNASer formation. 
Biochimie. 93 (2011), 10; 1761-1769
Dulic Morana; Pozar Josip; Bilokapic Silvija; Weygand-Durasevic Ivana; Gruic-Sovulj Ita.

Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases.
Croatica chemica acta. 84 (2011), 2; 149-157.
Mocibob Marko; Ivic Nives; Subasic Deni; Luic Marija; Weygand-Durasevic Ivana.

An Archaeal tRNA-Synthetase Complex that Enhances Aminoacylation under Extreme Conditions. 
The Journal of biological chemistry. 286 (2011), 5; 3396-3404
Godinic-Mikulcic Vlatka; Jaric Jelena; Hausmann Corinne D; Ibba Michael; Weygand-Durasevic Ivana.

Pre-transfer editing of serine hydroxamate within the active site of methanogenic-type seryl-tRNA synthetase. 
Croatica chemica acta. 84 (2011), 2; 179-184
Gruic-Sovulj Ita; Dulic Morana; Weygand-Durasevic Ivana.

2010

Partitioning of tRNA-dependent Editing between Pre- and Post-transfer Pathways in Class I Aminoacyl-tRNA Synthetases. 
The Journal of biological chemistry. 285 (2010), 31; 23799-23809
Dulic, Morana; Cvetesic, Nevena; Perona, John J.; Gruic-Sovulj, Ita.

Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis. 
Proceedings of the National Academy of Sciences of the United States of America. 107 (2010), 33; 14585-14590
Mocibob Marko; Ivic Nives; Bilokapic Silvija; Maier Timm; Luic Marija; Ban Nenad; Weygand-Durasevic Ivana.

Efficiently activated serine analogue is not transferred to yeast tRNASer. 
Croatica chemica acta. 83 (2010), 2; 163-169
Gruic-Sovulj Ita; Dulic Morana; Jaric Jelena; Cvetesic Nevena; Majsec Kristina; Weygand-Durasevic Ivana.

Differences between reversible (self-association) and irreversible aggregation of rHuG-CSF in carbohydrate and polyol formulations. 
European journal of pharmaceutics and biopharmaceutics. 76 (2010), 3; 357-365
Pavisic Renata; Dodig Ivana; Horvatic Anita; Mijic Lucija; Sedic Mirela; Rajic Linaric Masa; Gruic-Sovulj Ita; Preocanin Tajana; Bukvic Krajacic Mirjana; Cindric Mario.

Synthesis and Biological Activity of Mannose Conjugates with 1-Adamantamine and Ferrocene Amines. 
Croatica chemica acta. 83 (2010), 4; 421-431
Ribic Rosana; Kovacevic Monika; Petrovic Perokovic Vesna; Gruic-Sovulj Ita; Rapic Vladimir; Tomic Srdanka

2009

Identification of Amino Acids in the N-terminal Domain of Atypical Methanogenic-type Seryl-tRNA Synthetase Critical for tRNA Recognition.
The Journal of biological chemistry. 284 (2009), 44; 30643-30651
Jaric Jelena; Bilokapic Silvija; Lesjak Sonja; Crnkovic Ana; Ban Nenad; Weygand-Durasevic Ivana.

Idiosyncratic Helix-Turn-Helix Motif in Methanosarcina barkeri Seryl-tRNA Synthetase Has a Critical Architectural Role.
Journal of Biological Chemistry. 284 (2009), 16; 10706-10713
Bilokapic Silvija; Ivic Nives; Godinic-Mikulcic Vlatka; Piantanida Ivo; Ban Nenad; Weygand-Durasevic Ivana.

2008

Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition.
FEBS Journal. 275 (2008), 11; 2831-2844
Bilokapic Silvija; Rokov-Plavec Jasmina; Ban Nenad; Weygand-Durasevic Ivana.

Dual targeting of organellar seryl-tRNA synthetase to maize mitochondria and chloroplasts.
Plant Cell Reports. 27 (2008), 7; 1157-1168
Rokov-Plavec Jasmina; Dulic Morana; Duchene Anne-Marie; Weygand-Durasevic Ivana.

The proximal region of a noncatalytic eukaryotic seryl-tRNA synthetase extension is required for protein stability in vitro and in vivo. 
Archives of Biochemistry and Biophysics 470 (2008), 2; 129-138
Mocibob Marko; Weygand-Durasevic Ivana.

2007

Hydrolysis of non-cognate aminoacyl-adenylates by a class II aminoacyl-tRNA synthetase lacking an editing domain. 
FEBS Letters. 581 (2007), 26; 5110-5114
Gruic-Sovulj, Ita; Rokov-Plavec, Jasmina; Weygand-Durasevic, Ivana.

Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNA^Ser.
FEBS Journal. 274 (2007), 11; 2788-2799
Godinic Vlatka; Mocibob Marko; Rocak Sanda; Ibba Michael; Weygand-Durasevic Ivana.

2006

Structure of the unusual methanogen seryl-tRNA synthetase reveals a distinct zincdependent mode of substrate recognition. 
EMBO Journal. 25 (2006.), 11; 2498-509
Bilokapic Silvija; Maier Timm; Ahel Dragana; Gruic-Sovulj Ita; Söll Dieter; Weygand-Durasevic Ivana; Ban Nenad.

Shuffling of Discrete tRNASer Regions Reveals Differently Utilized Identity Elements in Yeast and Methanogenic Archaea. 
Journal of molecular biology. 361 (2006), 1; 128-139
Gruic-Sovulj Ita; Jaric Jelena; Dulic Morana; Cindric Mario; Weygand-Durasevic Ivana.

2005

tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase. 
The Journal of biological chemistry. 280 (2005.) ; 23978-23986
Gruic-Sovulj Ita; Uter Nathan; Bullock Timothy; Perona John.

Amino acid-dependent transfer RNA affinity in a class I aminoacyl-tRNA synthetase.
The Journal of biological chemistry. 280 (2005.); 23966-23977
Uter Nathan; Gruic-Sovulj Ita; Perona John.

Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.
FEBS letters. 579 (2005); 4344-4348
Ahel Dragana; Slade Dea; Mocibob Marko; Söll Dieter; Weygand-Durasevic Ivana.

2004

Stability of the complex between yeast seryl-tRNA synthetase and tRNASer under different electrophoretic conditions.
Croatica Chemica Acta. 77 (2004), 4; 1643-1650
Gruic-Sovulj Ita; Rokov-Plavec Jasmina; Mocibob Marko; Kamenski Tomislav; Weygand-Durasevic Ivana.

Unilateral flexibility in tRNASer recognition by heterologous seryl-tRNA synthetases.
Periodicum biologorum. 106 (2004); 147-154
Rokov-Plavec Jasmina; Bilokapic Silvija; Gruic-Sovulj Ita; Mocibob Marko; Glavan Filip; Brgles Marija; Weygand-Durasevic Ivana.

2002

tRNA-dependent amino acid discrimination by yeast sery-tRNA synthetases. 
European journal of biochemistry. 269 (2002) ; 5271-5279
Gruic-Sovulj Ita; Landeka Irena; Söll Dieter; Weygand-Durasevic Ivana.

Maize seryl-tRNA synthetase: specificity of substrate recognition by the organellar enzyme.
Archives of Biochemistry and Biophysics. 397 (2002), 1; 40-50
Rokov-Plavec Jasmina; Lesjak Sonja; Landeka Irena; Mijakovic Ivan; Weygand-Durasevic Ivana.

The accuracy of seryl-tRNA synthesis. 
Food technology and biotechnology. 40 (2002) ; 247-253
Weygand-Durasevic Ivana; Gruic-Sovulj Ita; Rocak Sanda; Landeka Irena.

2001

Influence of modified tRNA^Tyr on the activation of tyrosine catalyzed by tyrosyl-tRNA synthetase from Saccharomyces cerevisiae. 
Croatica Chemica Acta. 74 (2001), 1; 161-171
Gruic-Sovulj Ita; Weygand-Durasevic Ivana; Kucan Zeljko.

2000

Organellar and cytosolic seryl-tRNA synthetases: structural, functional and evolutionary aspects. 
Acta Biologica Slovenica. 43 (2000), 1-2; 109-116
Weygand-Durasevic Ivana; Lenhard Boris; Rocak Sanda; Rokov Jasmina; Landeka Irena; Mijakovic Ivan.

1999

Seryl-tRNA synthesis in maize. 
Periodicum biologorum. 101 (1999), 2; 137-142
Rokov Jasmina; Weygand-Durasevic Ivana.

1998

Maize mitochondrial seryl-tRNA synthetase recognizes Escherichia coli tRNA^Ser in vivo and in vitro.
Plant molecular biology. 38 (1998), 3; 497-502
Rokov Jasmina; Soll Dieter; Weygand-Durasevic Ivana.