dr. sc. Morana Dulić

Assistant Professor

Contact
Tel: +385 1 4606 235
Mail: mdulic[at]chem.pmf.hr

Education
2012. PhD degree, thesis on “Opposite catalytic activities within synthetic site of isoleucyl-tRNA synthetase from Escherichia coli”, under mentorship of prof. dr. sc. Ita Gruić-Sovulj, Division of Biochemistry, Department of Chemistry, University of Zagreb

2006. Diploma in Molecular Biology with a thesis on “Cellular localization of organellar seryl-tRNA synthetase in maize” under mentorship of prof. dr. sc. Ivana Weygand-Đurašević, Division of Biochemistry, Department of Chemistry, University of Zagreb

Research interests
Proofreading of aminoacyl-tRNA synthetases, enzyme kinetics, thermodynamics, molecular evolution.

Publications
Kinetic Origin of Substrate Specificity in Post-Transfer Editing by Leucyl-tRNA Synthetase.
Journal of Molecular Biology. 430 (2018), 1; 1-16
Dulic Morana; Cvetesic Nevena; Zivkovic Igor; Palencia Andrés; Cusack Stephen; Bertosa Branimir; Gruic-Sovulj Ita.

Discovery and Investigation of Natural Editing Function against Artificial Amino Acids in Protein Translation.
ACS Central Science. 3 (2017), 1; 73-80
Voeller Jan-Stefan; Dulic Morana; Gerling-Driessen Ulla I. M.; Biava Hernan; Baumann Tobias; Budisa Nediljko; Gruic Sovulj Ita; Koksch Beate

Naturally Occuring Isoleucyl-tRNA Synthetase without tRNA-dependent Pre-transfer Editing.
The Journal of biological chemistry. 291 (2016), 16; 8618-8631 
Cvetesic Nevena; Dulic Morana; Bilus Mirna; Sostaric Nikolina; Lenhard Boris; Gruic-Sovulj Ita.

Determinants for tRNA-dependent pre-transfer editing in the synthetic site of isoleucyl-tRNA synthetase.
Biochemistry. 53 (2014), 39; 6189-6198

Dulic Morana; Perona John Joseph; Gruic Sovulj Ita.

Substrate recognition and fidelity of maize seryl- tRNA synthetases.
Archives of biochemistry and biophysics. 529 (2013), 2; 122-130
Rokov-Plavec Jasmina; Lesjak Sonja; Gruic-Sovulj Ita; Mocibob Marko; Dulic Morana; Weygand-Durasevic Ivana.

An idiosyncratic serine ordering loop in methanogen seryl-tRNA synthetases guides substrates through seryl-tRNASer formation.
Biochimie. 93 (2011), 10; 1761-1769
Dulic Morana; Pozar Josip; Bilokapic Silvija; Weygand-Durasevic Ivana; Gruic-Sovulj Ita.

Pre-transfer editing of serine hydroxamate within the active site of methanogenic-type seryl-tRNA synthetase.
Croatica chemica acta. 84 (2011), 2; 179-184
Gruic-Sovulj Ita; Dulic Morana; Weygand-Durasevic Ivana.

Partitioning of tRNA-dependent Editing between Pre- and Post-transfer Pathways in Class I Aminoacyl-tRNA Synthetases.
The Journal of biological chemistry. 285 (2010), 31; 23799-23809
Dulic Morana; Cvetesic, Nevena; Perona John J; Gruic-Sovulj Ita.

Efficiently activated serine analogue is not transferred to yeast tRNASer.
Croatica chemica acta. 83 (2010), 2; 163-169
Gruic-Sovulj Ita; Dulic Morana; Jaric Jelena; Cvetesic Nevena; Majsec Kristina; Weygand-Durasevic Ivana.

Dual targeting of organellar seryl-tRNA synthetase to maize mitochondria and chloroplasts.
Plant Cell Reports. 27 (2008), 7; 1157-1168
Rokov-Plavec Jasmina; Dulic Morana; Duchene Anne-Marie; Weygand-Durasevic, Ivana.

Shuffling of Discrete tRNASer Regions Reveals Differently Utilized Identity Elements in Yeast and Methanogenic Archaea.
Journal of molecular biology. 361 (2006), 1; 128-139
Gruic-Sovulj Ita; Jaric Jelena; Dulic Morana; Cindric Mario; Weygand-Durasevic Ivana.


dr. sc. Aleksandra Maršavelski

Assistant Professor

Contact
Tel: +385 1 4606 241
Mail: aleksandra.marsavelski[at]chem.pmf.hr

Education
2017. PhD in Chemistry, “Computational investigation of the catalytic activity and selectivity of amine oxidases towards histamine and N-methylhistamine”, under supervision of Dr. Robert Vianello, Division of Biochemistry, Department of Chemistry, University of Zagreb

2011. MSc in Biochemistry, “Purification and biochemical characterization of sPLUNC2 protein from human saliva”, under supervision of Associate Professor Natalija Polović, Faculty of Chemistry, University of Belgrade.

2010. BSc in Biochemistry, “Influence of carboxymethylcellulose and gum arabic on the production of antibiotics and proteolytic enzymes in Streptomyces hygroscopicus” under supervision of Professor Miroslav Vrvić, Faculty of Chemistry, University of Belgrade.

Research interests
Computational biochemistry and enzymology.

Publications
Empirical Valence Bond Simulations Suggest a Direct Hydride Transfer Mechanism for Human Diamine Oxidase.
ACS Omega (2018) just accepted
Aleksandra Maršavelski; Dušan Petrović; Paul Bauer; Robert Vianello; Shina Caroline Lynn Kamerlin.

The Catalytic Dyad in the SGNH Hydrolase Superfamily: In-depth Insight into Structural Parameters Tuning Catalytic Process of Extracellular Lipase from Streptomyces rimosus.
ACS Chemical Biology (2017), 12: 1928–1936.
Ivana Leščić Ašler; Zoran Štefanić; Aleksandra Maršavelski; Robert Vianello; Biserka Kojić-Prodić.

What a difference a methyl group makes - the selectivity of monoamine oxidase B towards histamine and N-methylhistamine.
Chemistry - A European Journal (2017), 23: 2915–2925.
Aleksandra Maršavelski; Robert Vianello.

15N NMR Spectroscopy, X-ray and Neutron Diffraction, Quantum-Chemical Calculations, and UV/vis-Spectrophotometric Titrations as Complementary Techniques for the Analysis of Pyridine-Supported Bicyclic Guanidine Superbases.
Journal of organic chemistry, (2016), 81: 7612–7625.
Ryan J. Schwamm; Robert Vianello; Aleksandra Maršavelski; M. Ángeles García; Rosa M. Claramunt; Ibon Alkorta; Jaan Saame; Ivo Leito; Christopher M. Fitchett; Alison J. Edwards; Martyn P. Coles.

A novel antimicrobial target—expanded and revisited mode of action of pantothenamides.
RSC advances, (2016), 6: 44888-44895
Aleksandra Maršavelski.

The Quantum Nature of Drug-Receptor Interactions: Deuteration Changes Binding Affinities for Histamine Receptor Ligands.
PLOS ONE, (2016), 11(5):e0154002.
Mojca Kržan; Robert Vianello; Aleksandra Maršavelski; Matej Repič; Maja Zakšek; Kristina Kotnik; Estera Fijan; Janez Mavri.

Synthesis and characterization of ML and ML2 metal complexes with amino acid substituted bis(2-picolyl)amine ligands.
Dalton transactions, (2016), 45: 2845-2858.
Đani Škalamera; Ernest Sanders; Robert Vianello; Aleksandra Maršavelski; Andrej Pevec; Iztok Turel; Srećko I. Kirin.

Supramolecular Ionic-Liquid Gels with High Ionic Conductivity.
Chemistry - A European Journal (2015), 21: 12121–12128.
Aleksandra Maršavelski; Vilko Smrečki; Robert Vianello; Mladen Žinić; Andrea Moguš-Milanković; Ana Šantić.

The origin of specificity and insight into recognition between aminoacyl carrier protein and its partner ligase.
Physical Chemistry Chemical Physics (2015), 17: 19030-19038.
Aleksandra Maršavelski; Marko Močibob; Ita Gruić-Sovulj; Robert Vianello.

A single amino acid substitution affects the substrate specificity of the seryl-tRNA synthetase homologue.
Molecular BioSystems (2014), 10: 3207-3216.
Aleksandra Maršavelski; Sonja Lesjak; Marko Močibob; Ivana Weygand-Đurašević, Sanja Tomić.

Isolation, biochemical characterization and anti-bacterial activity of BPIFA2 protein.
Archives of Oral Biology (2014), 59: 302-309.
Vladimir Prokopović; Milica Popović; Uroš Anđelković; Aleksandra Maršavelski; Brankica Rasković; Marija Gavrović-Jankulović; Natalija Polović.

The Nobel Prize in Chemistry 2013.
Kemija u industriji (2013), 62: 11-12.
Marina Grabar Branilović; Aleksandra Maršavelski; Antonija Tomić; Sanja Tomić.


dr. sc. Marko Močibob

Assistant Professor

Contact
Tel: +385 1 4606 235
Mail: mocibob[at]chem.pmf.hr

Education
2010. PhD thesis on “Non-catalytic protein domains of eukaryotic seryl-tRNA synthetases”, under mentorship of prof. dr. sc. Ivana Weygand-Đurašević, Division of Biochemistry, Department of Chemistry, University of Zagreb

2002. Diploma in Chemistry, thesis on “Electrophoretic investigation of the complexes between seryl-tRNA synthetases and tRNASer” under mentorship of prof. dr. sc. Ivana Weygand-Đurašević, Division of Biochemistry, Department of Chemistry, University of Zagreb

Research interests
Marko has a strong background in protein biochemistry, analysis of protein:protein interactions, enzyme kinetics, methods of molecular biology, and basic knowledge of bioinformatics and sequence analysis. His interest is focused on homologs of aminoacyl-tRNA synthetases and their physiological role.

Publications
On the Mechanism and Origin of Isoleucyl-tRNA Synthetase Editing against Norvaline.
Journal of Molecular Biology. 2019, doi:10.1016/j.jmb.2019.01.029
Bilus Mirna; Semanjski Maja; Mocibob Marko; Zivkovic Igor; Cvetesic Nevena; Tawfik S. Dan; Toth-Petroczy Agnes; Macek Boris; Gruic Sovulj Ita

Seryl-tRNA Synthetases in Translation and Beyond
Croatica Chemica Acta. 89 (2016), 2; 261-276
Mocibob Marko; Rokov-Plavec Jasmina; Godinic-Mikulcic Vlatka; Gruic-Sovulj Ita.

The origin of specificity and insight into recognition between an aminoacyl carrier protein and its partner ligase.
Physical Chemistry Chemical Physics. 17 (2015), 29; 19030-19038
Marsavelski Aleksandra; Mocibob Marko; Gruic-Sovulj Ita; Vianello Robert.

A single amino acid substitution affects the substrate specificity of the seryl-tRNA synthetase homologue.
Molecular biosystems. 10 (2014), 12; 3207-3216
Marsavelski Aleksandra; Lesjak Sonja; Mocibob Marko; Weygand-Durasevic Ivana; Tomic Sanja.

Adaptation of Aminoacyl-tRNA Synthetase Catalytic Core to Carrier Protein Aminoacylation.
Structure. 21 (2013), 4; 614-626
Mocibob Marko; Ivic Nives; Luic Marija; Weygand-Durasevic Ivana.

Substrate recognition and fidelity of maize seryl-tRNA synthetases.
Archives of biochemistry and biophysics. 529 (2013), 2; 122-130
Rokov-Plavec Jasmina; Lesjak Sonja; Gruic-Sovulj Ita; Mocibob Marko; Dulic Morana; Weygand-Durasevic Ivana.

Substrate Recognition by Novel Family of Amino Acid:[Carrier Protein] Ligases.
Croatica chemica acta. 84 (2011), 2; 149-157.
Mocibob Marko; Ivic Nives; Subasic Deni; Luic Marija; Weygand-Durasevic Ivana.

Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and provide a link between ribosomal and nonribosomal peptide synthesis.
Proceedings of the National Academy of Sciences of the United States of America. 107 (2010), 33; 14585-14590
Mocibob Marko; Ivic Nives; Bilokapic Silvija; Maier Timm; Luic Marija; Ban Nenad; Weygand-Durasevic Ivana.

The proximal region of a noncatalytic eukaryotic seryl-tRNA synthetase extension is required for protein stability in vitro and in vivo.
Archives of Biochemistry and Biophysics 470 (2008), 2; 129-138
Mocibob Marko; Weygand-Durasevic Ivana.

Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNASer.
FEBS Journal. 274 (2007), 11; 2788-2799
Godinic Vlatka; Mocibob Marko; Rocak Sanda; Ibba Michael; Weygand-Durasevic Ivana.

Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.
FEBS letters. 579 (2005); 4344-4348
Ahel Dragana; Slade Dea; Mocibob Marko; Söll Dieter; Weygand-Durasevic Ivana.

Stability of the complex between yeast seryl-tRNA synthetase and tRNASer under different electrophoretic conditions.
Croatica Chemica Acta. 77 (2004), 4; 1643-1650
Gruic-Sovulj Ita; Rokov-Plavec Jasmina; Mocibob Marko; Kamenski Tomislav; Weygand-Durasevic Ivana.


Jasmina Rokov

dr. sc. Jasmina Rokov Plavec

Assistant Professor

Contact
Tel: +385 1 4606 232
Mail: rokov[at]chem.pmf.hr

Education
2004. PhD degree, thesis on “Maize organellar seryl-tRNA-synthetase: characterization in vitro and in vivo”, under mentorship of prof. dr. sc. Ivana Weygand-Đurašević, Division of Biochemistry, Department of Chemistry, University of Zagreb

1998. Diploma in Molecular Biology, thesis on “Cloning and expression of the gene for seryl-tRNA synthetase from maize Zea mays” under mentorship of prof. dr. sc. Ivana Weygand-Đurašević, Division of Biochemistry, Department of Chemistry, University of Zagreb

Research interests
Jasmina is interested in structure, function and evolution of aminoacyl-tRNA synthetases with emphasis on plant seryl-tRNA synthetases (SerRS). She studies intracellular localization of proteins, role of plant SerRS in cellular stress response and plans to identify and characterise SerRS protein interactors.

Publications
Arabidopsis seryl‐tRNA synthetase: the first crystal structure and novel protein interactor of plant aminoacyl‐tRNA synthetase
The FEBS Journal. 286 (2019), 3; 536-554.
Kekez Mario; Zanki Vladimir; Kekez Ivana; Baranasic Jurica; Hodnik Vesna; Duchene Anne-Marie; Anderluh Gregor; Gruic Sovulj Ita; Matković-Čalogović Dubravka; Weygand Durasevic Ivana; Rokov-Plavec Jasmina

Seryl-tRNA Synthetases in Translation and Beyond
Croatica Chemica Acta. 89 (2016), 2; 261-276
Mocibob Marko; Rokov-Plavec Jasmina; Godinic-Mikulcic Vlatka; Gruic-Sovulj Ita.

Exclusive cytosolic localization and broad tRNASer specificity of Arabidopsis thaliana seryl-tRNA synthetase.
Journal of plant biology. 59 (2016), 1; 44-54
Kekez Mario; Bauer Natasa; Saric Ela; Rokov-Plavec Jasmina.

Substrate recognition and fidelity of maize seryl- tRNA synthetases.
Archives of biochemistry and biophysics. 529 (2013), 2; 122-130

Rokov-Plavec Jasmina; Lesjak Sonja; Gruic- Sovulj Ita; Mocibob Marko; Dulic Morana; Weygand-Durasevic Ivana.

Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition.
FEBS Journal. 275 (2008), 11; 2831-2844

Bilokapic Silvija; Rokov-Plavec Jasmina; Ban Nenad; Weygand-Durasevic Ivana.

Dual targeting of organellar seryl-tRNA synthetase to maize mitochondria and chloroplasts.
Plant Cell Reports. 27 (2008), 7; 1157-1168
Rokov-Plavec Jasmina; Dulic Morana; Duchene Anne-Marie; Weygand-Durasevic Ivana.

Hydrolysis of non-cognate aminoacyl-adenylates by a class II aminoacyl-tRNA synthetase lacking an editing domain.
FEBS Letters. 581 (2007), 26; 5110-5114
Gruic-Sovulj Ita; Rokov-Plavec Jasmina; Weygand-Durasevic, Ivana.

Stability of the complex between yeast seryl-tRNA synthetase and tRNASer under different electrophoretic conditions.
Croatica Chemica Acta. 77 (2004), 4; 1643-1650
Gruic-Sovulj Ita; Rokov-Plavec Jasmina; Mocibob Marko; Kamenski Tomislav; Weygand-Durasevic Ivana.

Unilateral flexibility in tRNASer recognition by heterologous seryl-tRNA synthetases.
Periodicum biologorum. 106 (2004); 147-154

Rokov-Plavec Jasmina; Bilokapic Silvija; Gruic-Sovulj Ita; Mocibob Marko; Glavan Filip; Brgles Marija; Weygand-Durasevic Ivana.

Maize seryl-tRNA synthetase: specificity of substrate recognition by the organellar enzyme.
Archives of Biochemistry and Biophysics. 397 (2002), 1; 40-50
Rokov-Plavec Jasmina
; Lesjak Sonja; Landeka Irena; Mijakovic Ivan; Weygand-Durasevic Ivana.

Organellar and cytosolic seryl-tRNA synthetases: structural, functional and evolutionary aspects.
Acta Biologica Slovenica. 43 (2000), 1-2; 109-116
Weygand-Durasevic Ivana; Lenhard Boris; Rocak Sanda; Rokov Jasmina; Landeka Irena; Mijakovic Ivan.

Seryl-tRNA synthesis in maize.
Periodicum biologorum. 101 (1999), 2; 137-142
Rokov Jasmina; Weygand-Durasevic Ivana.

Maize mitochondrial seryl-tRNA synthetase recorgnizes Escherichia coli tRNASer in vivo and in vitro.
Plant molecular biology. 38 (1998), 3; 497-502
Rokov Jasmina; Soll Dieter; Weygand-Durasevic Ivana.